Hematology and serum chemistry profiles for giraffes are presented. Values for 39 blood samples, collected from 14 giraffes, were analyzed and evaluated for variations relating to differences in sex, age, and restraint mode. Sex-specific variations were found in WBC and neutrophil numbers, total protein, and alkaline phosphatase concentrations. Age-specific variations were found in hematocrit, hemoglobin, erythrocytes, banded neutrophil and basophil numbers, and in calcium, blood urea nitrogen (BUN), total bilirubin, alkaline phosphatase, and creatinine concentrations. Variations relating to differences in restraint mode used to collect the blood samples were found in calcium and uric acid concentrations.