Theileria parasites were detected in five apparently healthy free-ranging giraffes (Giraffa camelopardalis Linnaeus, 1758) captured for translocation on a game ranch located approximately 60 km south west of Lusaka. Giemsa-stained blood smears examined under a light microscope showed characteristic oval and rod shaped intra-erythrocytic piroplasms. Polymerase chain reaction (PCR) products targeted on the 18S rRNA gene showed characteristic bands of Theileria spp. The average number of infected blood cells per field examined by light microscopy was estimated at 48.6% (n=50,