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Species characterization in the genus pestivirus according to palindromic nucleotide substitutions in the 5′ untranslated region

The palindromic nucleotide substitutions (PNS) at the three variable loci (V1, V2, and V3) in the 5′ untranslated region (UTR) of the Pestivirus genome have been considered for taxonomical segregation of the species, through the evaluation of 533 strains. On the basis of qualitative and quantitative secondary structure characteristics, species have been identified within the genus, determining genetic distances between species isolates, clarifying borderline and multirelated sequences, and characterizing and clustering the Pestivirus strains showing unexpected genomic sequences. Nine genomic

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