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Whole genome sequence analyses of three African bovine rotaviruses reveal that they emerged through multiple reassortment events between rotaviruses from different mammalian species

Animal-to-human interspecies transmission is one of the evolutionary mechanisms driving rotavirus strain diversity in humans. Although quite a few studies emanating from Africa revealed evidence of bovine-to-human rotavirus interspecies transmission, whole genome data of African bovine rotavirus strains are not yet available. To gain insight into the complete genome constellation of African bovine rotaviruses, the full genomes of three bovine rotavirus strains were extracted from stool samples collected from calves, amplified using a sequence-independent procedure, followed by 4541 pyrosequencing. Strains

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Development of 11 microsatellite markers for Giraffa camelopardalis through 454 pyrosequencing, with primer options for an additional 458 microsatellites

Many wild giraffe populations are declining across Africa, with two subspecies listed by the IUCN as Endangered in the past 4 years. We developed 11 microsatellite markers from Giraffa camelopardalis angolensis in Etosha National Park, Namibia using 454 sequencing. In 70 individuals, the loci showed 2–4 alleles per locus and expected heterozygosities of 0.082–0.711. There were no significant deviations from Hardy–Weinberg equilibrium for any of the loci. Null allele frequencies were low (<3 %) across all loci. We present primer options for

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